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KMID : 1094720200250050787
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Biotechnology and Bioprocess Engineering
2020 Volume.25 No. 5 p.787 ~ p.794
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Medium Optimization and Proteome Analysis of Protease Production by Janthinobacterium sp.
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Kim Hyun-Do
Choi Jong-Il
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Abstract
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This study describes the optimization of a culture medium to improve protease production by Janthinobacterium sp. using response surface methodology (RSM) and comparative proteomic analysis. Using a one-variable-at-a-time approach, dextrose, Triton X-100, peptone, and pH were identified as significant factors affecting protease production. The optimized medium resulted in a 6.25-fold increase in protease activity when compared to that in the original medium. To investigate the metabolic changes in the optimized medium, extracellular and intracellular proteins were analyzed by two-dimensional gel electrophoresis. Some proteins essential to cell viability were highly expressed in the optimized medium and the outer membrane protein A was secreted at a higher level. These results can be useful for the industrial application of psychrophiles that produce cold-adapted enzymes.
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KEYWORD
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Antarctic microorganism, medium optimization, protease, proteome analysis
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